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Target information above from: Image courtesy of Human Protein Atlas. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Further results for this antibody can be found at www. Publishing research using ab? Please let us know so that we can cite the reference in this datasheet. I have 25314 back from the lab with the following explanation regarding the membrane vs.
From what we can gather this protein can also show cytoplasmic localisation swissprot. It’s very difficult to distinguish between cytoplasmic and membrane staining and we had very little data on 52134 antibody as to the expected staining pattern.
It seems to be consistent with other companies’ data. However, 225134 welcome any feedback on the staining pattern from the customer for this antibody in IF. Thank you for confirming these details and for your cooperation.
The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab To check the status of the order please contact our Customer Service 2513 and reference this number.
Please note that this 2134 of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. Thank you for your reply. 225134 free of charge vial of ab has been issued, which should arrive tomorrow. I accept your offer and I would fs to proceed and test ab in flow cytometry.
I wait the discount code and then I will order ab Thank you Best regards. October I am very pleased to hear you would like to accept our offer and test ab in flow cytometry. This code will give you the value of ab off a futuire order before the expiration date. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The sd will be active once the Abreview has been submitted and can be redeemed rs one of the dw ways: Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us.
We look forward to receiving your Abreview and wish you luck with your research. Ss terms and conditions applicable to this offer can be found here: In the data sheet there are: Thank you very much for your interest in ab To our knowledge,ab has not been 2534 in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab now, test it inflow cytometry and submit feedback to us in the form of an Abreview.
The discount would be to the value of ab off a future order. If you are interested in this offer, please follow vs steps: Reply to this e-mail to let me know that you would like to proceed and test ab in flow cytometry.
I will then send a discount code. This code must be issued before purchasing ab so please wait for my reply before ordering. Purchase ab either by phone, fax, or online es. Test it in flow cytometry. Let us know the results, positive or negative, using our Abreview system this will take about 10 minutes and images are great if you have them!
Anti-HCP1 / PCFT antibody (ab25134)
To find out how to submit an Abreview, please visit: After the review is submitted to us, ss discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code.
The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab turns out to be unsuitable for flow cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: If there is not another lot available, then we would like a credit for the HCP1 antibody. Thank you for your continual support with this issue. Thank you for contacting us.
I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department.
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To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.
Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing 21534 products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice. We have now tested the blocking peptide with the HCP1 antibody.
Those lysates are now picking up several non-specific bands as well. Would you be able to provide another lot of the HCP1 antibody for our 251134
Thank you for following up with your results. I am sorry that the blocking peptide experiments did not demonstrate the specificity of the bands you were detecting in your samples. I vs checked our available stock and we only have the lot you currently have available. 251344 would be happy to offer you another vial from this lot or a credit.
It seems that the other antibodies to this target have not been validated for WB and mouse. I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions. Thank you for your fast response.
We have continued to troubleshoot at our end as well. Attached is an immunoblot with mouse kidney and LNCaP cell lysates. Do you know if the antibody that you have provided is from the same batch as in the figure on your website with mouse kidney as a positive control? Would you be able to provide the peptide ab so that we could confirm positive signal? The new results you obtained are quite interesting.
The image on our website is not representing data from the current lot, but is representative of the expected results with this antibody. Per your request, I have sent you the HCP1 peptide ab free of charge. This should arrive to you early next week. I hope this will be helpful for your experiments. I am using this antibody to detect HCP1 in mouse kidney samples and detecting a band at around 75kDa.
Have you observed this before? Thank you for contacting Abcam regarding ab I have asked the lab to retest this antibody. If you would provide an order number I would be happy to offer a replacement or credit. Please let me know which you would prefer. Please do not hesitate to contact us if you have any additional questions.